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C-myc epitope tag antibody

WebBioLegend's Posi-Tag Epitope Tag Protein can be utilized as a positive control in Western blotting experiments where many epitope tags are commonly used. Posi-Tag represents proteins fused in the following order: GST, T7, HSV, c-Myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag, HA, and 6-His, and has an approximate molecular weight of 45 kDa. WebICC staining of purified anti-c-Myc antibody (clone 9E10) on HeLa cells transfected with multi-tag plasmid containing c-Myc epitope sequence. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100, and blocked with 5% fetal bovine serum in PBS for 1 hour.

Overview of Epitope Tagging Thermo Fisher Scientific - US

WebThe c-myc tag is derived from the c-terminal of the c-myc gene and it can be effectively recognized by antibodies. As such, it is a widely used tag for the detection of proteins in … WebPurified anti-c-Myc Antibody - The c-myc protein is a 62 kD nuclear factor that is ubiquitously expressed in the nucleus. ... (250 µg total protein) prepared from HeLa cells … illinois recreational therapy association https://nicoleandcompanyonline.com

MYC Epitope Tag Antibody (600-401-381) Rockland

WebA myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity … WebAnti-c-Myc Antibody (HRP) (A63720) ELISA, WB. HRP. Anti-c-Myc Antibody [9E10] (A85464) FC, IP, WB, IHC-P. Unconjugated. Anti-c-Myc Antibody [9E10] (Biotin) … WebMyc Tag Antibodies. The Myc tag, derived from the c-Myc protein, is a popular epitope tag for detecting the expression of recombinant proteins in yeast, bacteria, insect, and mammalian cell systems. The Myc tag may be fused to either the N-terminus or C … Find the Myc Tag antibody that fits your needs. Choose from 1 of 77 Myc Tag … illinois recreational pot shops

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C-myc epitope tag antibody

HRP Anti-Myc tag antibody [9E10] (ab62928) Abcam

WebMyc Tag Monoclonal Antibody (Myc.A7), DyLight™ 488, Invitrogen™-Mouse Monoclonal Antibody Achetez Myc Tag Monoclonal Antibody (Myc.A7), DyLight™ 488, Invitrogen™ WebThe monoclonal Anti-c-myc antibody facilitates the fast and convenient detection of c-myc-tagged fusion proteins, expressed in either prokaryotic or eukaryotic cells. The antibody is available conjugated with horseradish peroxidase (HRP) or fluorochrome- or biotin-conjugated for fast and convenient analysis of c-myc-tagged fusion proteins.

C-myc epitope tag antibody

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WebAnti-Myc Tag Antibody, clone 4A6 Anti-Myc Tag, clone 4A6, is a mouse monoclonal antibody that is validated for use in ChIP, IC, IF, IP, and WB for the detection of Myc Tag and does not show the very high context sensitivity of 9E10. This antibody has been cited in over 480 publications. - Find MSDS or SDS, a COA, data sheets and more information. WebThe mouse Anti-c-MYC monoclonal antibody recognizes an epitope located within a sequence (EQKLISEEDL) of the product of the human oncogene c-myc. c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene.

WebSep 27, 2024 · Epitope tags are widely used in various applications, but often lack versatility. ... Ramsay, G. & Bishop, J. M. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product ... WebJan 28, 2024 · We tested by Western blotting six antibodies that recognize the c-Myc epitope tag, including monoclonal antibodies 9E10, 4A6, 9B11, and 71D10 and …

WebWhat are epitope tags? Antibodies are an essential tool to determine the localization and expression of target proteins, and for purification in the presence of numerous other proteins in living organisms. ... c-Myc protein is a transcription factor involved in the cell cycle and apoptosis. Abnormalities in c-Myc, such as mutations and ... WebWe offer a wide range of validated antibodies for epitope tags. ️ High Quality Products ️ Low Prices ️ 100% Guarantee. Useful Links. 01223 298 875; ... Mouse monoclonal …

Web(B) 9E10 antibody, which specifically recognizes the human myc epitope tag and does not cross-react with murine c-myc, was used to immunoprecipitate myc-tagged ΔN89β-catenin from extracts of the ...

WebMagnetic beads were coupled to an anti-c-myc antibody and exposed to a mixture of H1N1 and the biotinylated c-myc-tagged 238D2 (AviTag-238D2, lane 1), or the biotinylated c-myc-tagged 3SM5 (AviTag-3SM5, lane 2) or to H1N1 alone (lane 3). In lanes 4, 5, and 6 are shown the inputs. The beads eluate, and the input samples were analyzed by Western ... illinois recruiting classWebWe offer an extensive range of high performance great value epitope tag antibodies and anti-reporter protein antibodies. Our range includes V5 tag antibodies, His-6 tag … illinois recruiting class 2021WebThe 9E10 antibody epitope (EQKLISEEDL) derives from a protein sequence in the human proto-oncogen p62(c-myc) and is widely used as a protein fusion tag. This myc-tag is a powerful tool in protein localization, immunochemistry, ELISA or protein purification. Here, we characterize the myc-tag epitope by substitutional analysis and length ... illinois recruiting class 2022WebImmunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide. The sequence corresponds to amino acids 410-419 of human c-Myc. illinois recreation parks associationWebMar 22, 2024 · One of the most widely used epitope tags is the myc-tag, recognized by the anti-c-Myc hybridoma antibody Myc1-9E10. Combining error-prone PCR, DNA … illinois recreation therapy associationWebPurified anti-c-Myc Antibody - The c-myc protein is a 62 kD nuclear factor that is ubiquitously expressed in the nucleus. ... (250 µg total protein) prepared from HeLa cells mock-transfected (-) or transfected (+) with multi-tag plasmid containing c-Myc epitope sequence were immunoprecipitated overnight with 2.5 µg of purified mouse IgG1, κ ... illinois recreational weed storesWebMyc Tag Monoclonal Antibody (Myc.A7), DyLight™ 488, Invitrogen™-Mouse Monoclonal Antibody Achetez Myc Tag Monoclonal Antibody (Myc.A7), DyLight™ 488, Invitrogen™ illinois recruiting class football