Nsp4 toxin
Web25 okt. 2005 · Serum anti-NSP4 IgG2a isotype titers were substantially greater than IgG1 titers, suggesting a dominant Th1 immune response. ELISA measurement of cytokines … Web25 okt. 2005 · A fusion protein containing the shiga toxin-1 B subunit (STB) linked to a 90 amino acid peptide (aa residues 86--175) from simian rotavirus (SA--11) nonstructural protein NSP4 was synthesized in Escherichia coli. Mice orally inoculated with 60 microg of STB::NSP4(90) fusion protein per dose generate …
Nsp4 toxin
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Web24 jan. 2006 · Biologically active recombinant NSP4(90)-RTB fusion protein was shown to bind glycoprotein asialofetuin receptor molecules in an in vitro enzyme-linked … Web24 jan. 2006 · A novel targeted fusion toxin protein with full biological activity is reported, which reacted poorly with asialofetuin, but bound strongly to IL-2 receptor based on …
Web3 mrt. 2024 · The NSP4 VPD channel was capable of conducting K +, Ca 2+, and Ba 2+ ions and shows a strong selectivity for cations over anions, but interestingly, it was not selective for Ca 2+ over K + ions ... Web20 dec. 2011 · Background Nonstructural glycoprotein 4 (NSP4) encoded by rotavirus is the only viral protein currently believed to function as an enterotoxin. NSP4 is synthesized as an intracellular transmembrane glycoprotein and as such is essential for virus assembly. Infection of polarized Caco-2 cells with rotavirus also results in the secretion of …
Web21 sep. 2005 · Europe PMC is an archive of life sciences journal literature. The castor-oil plant Ricinus communis A-B dimeric toxin B subunit (RTB) was genetically linked at its N-terminus with a 90 amino acid peptide from simian rotavirus SA-11 non-structural protein NSP4(90) and produced in Escherichia coli BL21 cells. Web31 mrt. 2005 · Rotavirus NSP4: A Multifunctional Viral Enterotoxin Viral Immunology Viral Immunology Vol. 18, No. 1 Special Focus Section: Enteric Viruses Rotavirus NSP4: A Multifunctional Viral Enterotoxin Dr. Judith M. Ball , Deanne M. Mitchell , Thomas F. Gibbons , and Rebecca D. Parr Published Online: 31 Mar 2005 …
Web1 okt. 2005 · Europe PMC is an archive of life sciences journal literature. A fusion protein containing the shiga toxin-1 B subunit (STB) linked to a 90 amino acid peptide (aa residues 86--175) from simian rotavirus (SA--11) nonstructural protein NSP4 was synthesized in Escherichia coli.
Web20 dec. 2011 · PDF Nonstructural glycoprotein 4 (NSP4) encoded by rotavirus is the only viral protein currently believed to function as an enterotoxin. NSP4 is... Find, read … how to yell at people in notorietyWebThe Shiga toxin (a non-pore forming toxin) is transferred to the cytosol via Golgi network and endoplasmic reticulum (ER). From the Golgi toxin is trafficked to the ER. Shiga toxins act to inhibit protein synthesis within … orkney road karrathaWebproteins. Nonstructural protein 4 (NSP4) is the entero-toxin of this virus, which plays its role in the villus epithelial cells and causes diarrhea (2). The non-structural protein 4 (NSP4) … how to yell in notorietyWebHigher Expression Level and Lower Toxicity of Genetically Spliced Rotavirus NSP4 in Comparison to the Full-Length Protein in E. coli Authors Mehdi Sahmani 1 , Siavash … how to yell medic in tf2WebNSP4 and the synthetic peptide NSP4 114–135 have been demonstrated to cause diarrhea in rodents aged 10–14 days [3, 27]. Infected intestinal cells release NSP4 into the intestinal lumen, where it acts as a viral toxin inducing Ca2+-dependent Cl− secretion, and inhibition of the Na+/glucose-cotransporter SGLT1 [13, 27]. Thus, glucose orkney resortsWebA transmembrane glycoprotein, NSP4 is organized into three main domains: a three-helical TM domain in the N-terminus (also a viroporin domain), a central cytoplasmic coiled-coil domain for multimerization, and an C-terminal flexible region. It can also be secreted … orkney restaurants with roomsWebRotavirus toxin NSP4 induces diarrhea by activation of TMEM16A and inhibition of Na+ absorption. Rotavirus infection is the most frequent cause for severe diarrhea in infants, … orkney riding of the marches