WebOct 21, 2024 · TIR1/AFBs is an F-box protein and a component of SKP1, Cullin, and F-box complex (SCF), which is involved in protein degradation mediated by the proteasome. The auxin/indole-3-acetic acid (Aux/IAA) protein is a specific substrate of the SCF TIR1/AFBs complex that negatively regulates auxin signal transduction. WebThe mechanism by which auxin regulates the plant SCF‐TIR1 complex strongly suggests that developing small molecule compounds as ubiquitin ligase agonists represents a new …

Phosphate Availability Alters Lateral Root Development in

WebJan 10, 2012 · This molecule was used as the basis for the development of a more potent TIR1 antagonist, auxinole (α-[2,4-dimethylphenylethyl-2-oxo]-IAA), using a structure-based … WebMar 1, 2008 · The auxin receptor TIR1 is an F-box protein functioning in a ubiquitin ligase complex to target repressors for degradation. It is itself an unstable protein, but newly … react draggable handle

Characterization of the Last Subunit of the Arabidopsis COP9 ...

The plant hormone auxin binds Tir1 (Transport Inhibitor Response 1). Tir1 is an Auxin Signaling F-box Protein (AFB) that acts as an auxin receptor. Auxin-bound Tir1 stimulates binding of SCF-Tir1 to the AUX/IAA repressor. Subsequent degradation of the repressor results in activation of AUX/IAA (i.e. auxin … See more Skp, Cullin, F-box containing complex (or SCF complex) is a multi-protein E3 ubiquitin ligase complex that catalyzes the ubiquitination of proteins destined for 26S proteasomal degradation. Along with the anaphase-promoting complex See more SCF contains a variable F-box protein and three core subunits: • F-box protein (FBP) – FBP contributes to the substrate … See more The eukaryotic cell cycle is regulated through the synthesis, degradation, binding interactions, post-translational modifications of regulatory proteins. Of these regulatory … See more The first hint that led to the discovery of the SCF complex came from genetic screens of Saccharomyces cerevisiae, also known as budding yeast. Temperature … See more Recently, SCF complexes have become an attractive anti-cancer target because of their upregulation in some human cancers and their biochemically distinct active sites. Though many … See more WebAug 17, 2012 · Our results strongly suggest that IAA8 would be degraded by the auxin-mediated interaction with TIR1/AFB receptors, which are components of the SCF TIR1/AFB ubiquitin-ligase complex, like other Aux/IAA proteins in the nucleus. Next, we conducted the same assays to examine the protein-protein interaction between IAA8 and ARF … WebIn the SCF complex, it serves as an adapter that links the F-box protein to CUL1. SCF(UFO) is required for vegetative and floral organ development as well as for male gametogenesis. … react draggable object